Medicinal plants are traditionally used in the treatment of human infections. The present study was carried out to compare the potential of Annona senegalensis and Cassia singueana root extracts against Proteus mirabilis related infections. The crude methanol root extracts were subjected to disc diffusion method to determine the activity of the crude methanol extracts. The methanol extracts of the two medicinal plants were further fractionated using solvents of different polarity (n-Hexane, ethyl acetate and n-butanol). The fractions were further subjected to antibacterial activity using same disc diffusion method in which Cassia singueanae thylacetate fraction prove to be the most active fraction, and no activity was observed in n- hexane fraction of the two extracts.